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PAK4 (p21 Activated Kinase 4, CDKN1A, Activated Kinase 4, Serine/Threonine Kinase PAK 4)

Cat no: P2488-24K

PAK4 (p21 Activated Kinase 4, CDKN1A, Activated Kinase 4, Serine/Threonine Kinase PAK 4)

The PAK (p21-activated kinase) family of serine/threonine kinases plays an important role in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, etc. Several mechanisms that induce PAK activation have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. More recently identified members PAK4, PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the regulatory N-terminal region. It has been demonstrated that phosphorylation of serine 474 of PAK4 may play a pivotal role in the activity and function of PAK4 kinase\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nWestern Blot: 1ug/ml\nImmunoprecipitation: 20ug/ml\nImmunohistochemistry: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: P2488-24K1\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P2488-24K

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by antigen affinitiy chromatography.

Isotype

IgG

Additional Info

Recognizes human PAK4 at ~72kD. The antibody does not cross-react with other Paks. Species crossreactivity: mouse, rat

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