PALS (Protein Associated with LIN7-1, PALS1, FLJ12615, MAGUK p55 Subfamily Member 5, Membrane Protein Palmitoylated 5, MPP5, Stardust (Drosophila))

MAGUKs are scaffold proteins that interact with cytoskeleton and regulate cell proliferation, signaling pathways and intracellular junctions and associate in tumor suppression and receptor clustering. PALS1, a 675aa protein, is a member of p55-like subfamily of MAGUKs. It is a human ortholog to Drosophila Stardust and mouse homolog of protein associated with LIN7. It consists of central SH3 domain, C-terminal PDZ and GUK domains, and an L27domain. PALS1 is targeted to tight junction with its L27 domain and plays a significant role in tight-junction protein targeting, vesicle trafficking and establishment and maintenance of cell polarity in polarized cells like mammalian neurons and epithelial cells. In Drosophila, PALS1 interacts with PATJ and PAR6 binding sites and forms 2 evolutionary conserved multi-protein complexes viz., Crumbs-PALS1-PATJ and Cdc42-Par6-Par3, implicated in the assembly of tight junctions. Mutations in PALS1 drosophila homolog caused polarity defects in Drosophila epithelia.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry (paraffin): 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Brain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P2817-50A

Size

100ug

Applications

IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kamberov E, Makarova O, Roh M, Liu A, Karnak D, Straight S, Margolis B. Molecular cloning and characterization of Pals, proteins associated with mLin-7. J Biol Chem. 2000 Apr 14;275(15):11425-31. PMID: 10753959. 2. MH Roh et.al The Journal of Cell Biology 157 (1); 161-172 (April 1, 2002). 3. Q Wang et.al J. Biol. Chem., 279 (29); 30715-30721 (July 16, 2004).

Additional Info

Recognizes human PALS.