Pannexin-2 (Panx2 and Px2)

Gap junctions are formed by a hexameric group of proteins called connexions for the transport of low molecular weight proteins from cell to cell. Connexins, which are present in all metazoan organisms, serve diverse functions ranging from control of cell growth and differentiation to electric conduction in excitable tissues. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. The pannexin gene family encodes a second class of putative gap junction proteins. Pannexins are highly conserved in invertebrates and mammals, indicating the importance of their gap junctional coupling function. Mammalian Pannexin-1 and Pannexin-3 are closely related, while Pannexin-2 is more distantly related. Pannexin-2 is a transmembrane protein expressed in the central nervous system that is unable to assemble in homomeric channels but forms heteromeric channels with Pannexin-1.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of Pannexin-2 in 20ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody.\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167602

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Bruzzone R., et al. (2003) Proc. Natl. Acad. Sci. USA 100: 13644-13649.\n2. Bao L., et al. (2004) FEBS Lett. 572: 65-68.\n3. Baranova A., et al. (2004) Genomics 83: 706-716.\n4. Vogt A., et al. (2005) Brain Res. Mol. Brain Res. 141:\n113-120.\n5. Bruzzone R., et al. (2005) J. Neurochem. 92: 1033-\n1043.\n6. Panchin Y.V. (2005) J. Exp. Biol. 208 (Pt. 8): 1415-\n1419.\n7. S

Additional Info

Recognizes rat Pannexin-2. Species Crossreactivity: Rat. Detects ~70kD. Does not cross-react with Pannexin-1 or Pannexin-3.