Paxillin, phosphorylated (Ser17)

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding \ndomains that participate in a variety of protein-protein interactions with kinases, GTPase- \nactivating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both \ntyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth- \nfactor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine \n178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes \nand epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during \ncell migration. \n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:500\nELISA: 1:2000\nOptimal dilution to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P3113-87C

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

RELATED REFERENCE: 1. Huang, C. et al. (2003) Nature 424:219-223. Huang, C. et al. (2004) Cell Cycle 3(1):4-6. 2. Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.

Additional Info

Detects a 68kD protein corresponding to the molecular weight of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells. Epitope: phosphoserine 178. Species Cross Reactivity: Human. This human sequence is highly conserved in rat and mouse paxillin.