PCDH18 (Protocadherin 18, Protocadherin-18, KIAA1562, DKFZp434B0923)

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 18 (PCDH18) was initially identified through screening of human and mouse ESTs using conserved cytoplasmic domain motifs. Tissue screening revealed PCDH18 broad expression in tissues such as brain, liver, heart, kidney, lung, and trachea. PCDH18 expression is also temporally and spatially regulated in the mouse embryonic brain and interacts with Disabled-1, an intracellular adapter protein involved in neuronal migration and cell positioning during mammalian brain development. PCDH18 was also found to be expressed in the developing zebrafish neural tube and central nervous system, lending support to the hypothesis that PCDH18 may play a role during brain development. At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRaji Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146406

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol. 2002; 14:557-62. 2. Wolverton T and Lalande M. Identification and characterization of three members of a novel subclass of protocadherins. Genomics 2001; 76:66-72. 3. Homayouni R, Rice DS, and Curran T. Disabled-1 interacts with a novel developmentally regulated protocadherin. Biochem. Biophys. Res. Commun. 2001; 289:539-47. 4. Kubota F, Murakami T, Tajika Y, et al. Expression of protocadherin 18 in the CNS and pharyngeal arches of zebrafish embryos. Int. J. Dev. Biol. 2008; 52:397-405.

Additional Info

Recognizes human PCDH12. Species Crossreactivity: mouse and rat.