PCNA (Proliferating Cell Nuclear Antigen) (FITC)

Proliferating cell nuclear antigen (PCNA), formerly known as cyclin, is a 36kD intranuclear acidic protein closely associated with proliferating cells. PCNA was originally identified in patients with systemic lupus erythematosus(1). It represents a component of DNA polymerase-b(2). The presence of PCNA is closely associated with the cell cycle. PCNA is expressed in high concentration in the late G1-phase before the DNA synthesis, and it reaches a maximum concentration in the S-phase(3). The level of PCNA declines during the G2 and M phases. \n\nApplications:\nFlow Cytometry: Neat-1:10. Use 5ul of the suggested working dilution to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher.\n\nRecommended Negative Controls: \nMouse IgG2a Negative Control: FITC\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3115-12D

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Matthews, M.B. et al. (1984). Identity of the proliferating cell nuclear antigen and cyclin. Nature 309: 374-376.2. Ogata, K. et al. (1985). Purification and N-terminal amino acid sequence of proliferating cell nuclear ant

Additional Info

Recognizes the proliferating cell nuclear antigen (PCNA), a nuclear protein vital for cellular DNA synthesis. Species Crossreactivity: Insects and all vertebrates.