PDCD4 (Programmed Cell Death Protein 4, Neoplastic Transformation Inhibitor Protein, Nuclear Antigen H731-like, Protein 197/15a, H731)

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nA-20 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3120-68L

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Jin Z, et al (2004) Cancer Biol. Ther. 4:139-63. 2. Shibihara K, et al (1995) Gene. 166:297-301. 3. Goke R, et al (2004) Am. J. Physiol. Cell Physiol. 287:C1541-6. 4. Yang HS, et al (2003) Mol. Cell Biol. 23:26-37. 5. Yang HS, et al (2001) Oncogene. 20:669-76. 6. Palamarchuk A, et al (2005) Cancer Res. 65:11282-6.

Additional Info

Recognizes human PDCD4. Species Crossreactivity: mouse and rat.