logo
logo
PDE7B Assay Kit, BioAssay(TM)

Cat no: 137350

PDE7B Assay Kit, BioAssay(TM)

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE7B is highly expressed in the pancreas and in other various tissues. Inhibition of PDE7B leads to induction of apoptosis.\n\nIntended Use:\nThe PDE7B Assay Kit is designed for identification of PDE7B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE7B to the binding agent.\n\nPhosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled?cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide?product. This increases the size of the nucleotide relative to unreacted cyclic?monophosphate. In the polarization assay, dye molecules with absorption transition?vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes?attached to the rapidly-rotating cyclic monophosphates will obtain random orientations?and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead?complexes will not have time to reorient and therefore will emit highly polarized light.\n\nTest Principle:\nThe PDE7B inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE7B enzyme, fluorescently labeled PDE7B substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE7B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE7B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE7B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.\n\nKit Components:\nNUNC microtiter plate, 1x96wells\nRecombinant enzyme, 1x1ug\nFAM-Cyclic-3

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

137350

Size

96Tests

References

1. Hetman, J., et al., PNAS. 97(1): 472-476 (2000). 2. Ikeda, M., et al., Biol. Psychiatry. 67(3): 263-269 (2010).

Read more on Supplier website
Advertisehere
BMA Biomedicals Horizontal banner
BMA Biomedicals

Latest promotion FROM:

BMA Biomedicals
View promotion

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...