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PEPCK Polyclonal Antibody

Cat no: 10004943

PEPCK Polyclonal Antibody

Antigen: mouse and rat PEPCK protein amino acids 5-17 (LHNGLDFSAKVIQ) . Host: rabbit . Cross Reactivity: (+) mouse and rat PEPCK protein . Application(s): WB . PEPCK catalyzes the conversion of oxaloacetate to phosphoenolpyruvate, the first committed step in gluconeogenesis. Both non-insulin-dependent diabetes mellitus and streptozotocin-induced diabetes result in elevated PEPCK activity, protein, and mRNA. This antibody was generated against mouse cytosolic PEPCK.

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SPECIFICATIONS

Catalog Number

10004943

Applications

WB

Hosts

Rabbit

Form

1 ea

P Type

Antibodies

Weight

0

Antigen

mouse and rat PEPCK protein amino acids 5-17 (LHNGLDFSAKVIQ)

Storage Temp

-20

Shipping Temp

-20

Additional Info

Phosphoenolpyruvate carboxykinase (PEPCK) is a hormonally regulated enzyme responsible for the first committed step in gluconeogenesis, catalyzing the conversion of oxaloacetate to phosphoenolpyruvate. PEPCK mRNA is most abundant in the liver, kidney cortex, and white adipose tissue but is also found in brown fat, the mammary gland during lactation, and the small intestine. Both non-insulin-dependent diabetes mellitus and streptozotocin-induced diabetes result in elevated PEPCK activity, protein, and mRNA. Two isozymes of PEPCK have been identified, cytosolic and mitochondrial. Cayman's PEPCK polyclonal antibody was generated against murine cytosolic PEPCK and detects the protein at 63 kDa by western blotting in rat and murine samples.

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