Perfecta3D(R) Cell Viability Kit

Cayman's Perfecta3D(R) Cell Viability Kit is a hanging drop cell culture format (developed by 3D Biomatrix) in which cells form spheroids that do not come into contact with plastic. Cell spheroids grown in the hanging drops can be maintained and their growth (with or without test compounds) can be monitored over a period up to two weeks. Cell proliferation is monitored by the enzymatic cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases in viable cells.

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SPECIFICATIONS

Catalog Number

600990

Form

5 ea

P Type

Assay Kits

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs. These have been routinely done in two dimensional (2D) culture plates with monolayer cells. There is a growing trend to replace 2D cell culture with 3D cell culture, because the latter better mirrors the environment experienced by normal cells in the body. One such 3D culture format is hanging drop cell culture (developed by 3D Biomatrix) in which cells form spheroids that do not come into contact with plastic. Cell spheroids grown in the hanging drops can be maintained and their growth with or without test compounds can be monitored over a period up to two weeks. Cayman's Perfecta3D(R) Cell Viability Kit provides an easy to use format for studying cell proliferation in the hanging drop model using a 3D cell culture plate. The assay is based on the enzymatic cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases in viable cells. This kit will allow investigators to screen drug candidates involved in cell cycle regulation in a 3D format.