Phosphodiesterase 9A2, Recombinant, Human, GST-Tag (PDE9A2)

Human PDE9A2 (GenBank Accession No. NM_001001567), full length with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. \n\nSpecific Activity: \n~90U/ug. 1 unit will lease 1 pmole phosphate per minute from 400uM 5'- GMP at 30 degrees C in a reaction buffer of 10mM Tris-HCl, pH 7.4, 1mM MgCl2. \n\nAssay Buffer: \n10mM Tris-HCl, pH 7.4, 1mM MgCl2; 0.4mM 3', 5'-cGMP substrate, 5ng/ul\nPDE9A.\n\nApplications: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

P4072-09B

Size

10ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 5mM glutathione, 3mM DTT, 30% glycerol.

Purity

~75% by SDS-PAGE

References

1. Rentero,C. and Puigdomenech,P BMC Mol. Biol. 7, 39 (2006) 2. Huai,Q., Wang,H., Zhang,W., Colman,R.W., Robinson,H. and Ke,H. JOURNAL Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9624-9629 (2004)