Phosphoinositide 3-Kinase delta, p110 delta, p85 alpha, Recombinant, Human (PI3K)

The class IA PI3K is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3K in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgEinduced degranulation and cytokine release. In neutrophils a role for PI3K in TNF -induced signalling was demonstrated by a reduction in Akt phosphorylation and PDK1 activity upon treatment with the specific inhibitor IC87114. The PI3K catalytic and regulatory subunits are coexpressed in Sf9 insect cells. The catalytic subunit carries a GST-Tag and the heterodimer was purified by affinity chromatography. The GST-Tag facilitates the protein

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P4073-34E

Size

10ug

Form

Liquid. Supplied in 25mM Tris, pH 8.0, 50mM NaCl, 0.5mM MgCl2 and 50% glycerol.

Purity

(same/more than) 95% by SDS-PAGE

References

Vanhaesebroeck et al. (1997) p110delta, a novel phosphoinositide 3-kinase in leukocytes. Proc. Natl. Acad. Sci. U.S.A. 94:4330.\nVanhaesebroeck et al. (1999) Autophosphorylation of p110 phosphoinositide 3-kinase: a new paradigm for the regulation of lipid kinases in vitro and in vivo. EMBO J. 18:1292.\nSadhu et al. (2003 ) Essential role of phosphoinositide 3-kinase delta in neutrophil directional movement. J Immunol. 70:2647.\nSawyer et al. (2003) Regulation of breast cancer cell chemotaxis by the phosphoinositide 3-kinase p110delta. Cancer Res. 63:1667.\nZhang et al. (2002) Human platelets contain p110delta phosphoinositide 3-kinase. Biochem Biophys Res Commun. 296:178.\nFoster et al. (2003) The phosphoinositide (PI) 3-kinase family. J. Cell Science 116:3037.\nAli et al. (2004) Essential role for the p110 phosphoinositide 3- kinase in the allergic response. Nature 431:1007.\nPuri et al. (2004) Mechanisms and implications of phosphoinositide 3-kinase in promoting neutrophil trafficking into inflamed tissue. Blood 103:3448.