Phosphoserine Phosphatase, Recombinant, Human (PSP)

Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+-dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the a-helical bundle domain, is important to substrate recognition and hydrolysis. Recombinant human hPSP was overexpressed in E. coli. It consists of 225 amino acids.\n\nSequence:\nMVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA \nALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA \nSKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG \nATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEE\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P4076-95

Size

50ug

Form

Supplied as a liquid in 20mM HEPES, pH 7.5, 1mM DTT, 100mM potassium chloride.

Purity

> 95% by SDS-PAGE. Purified by using conventional chromatography techniques.

References

1. Moro-Furlani., A.M., et al., Ann. Hum. Genet. 43: 323-333 (1980). 2. Collet, J.F., et al., FEBS Lett. 408: 281-284 (1997). 3. Kim, H.Y., et al., J. Biol. Chem. 277: 46,651-46,658 (2002).