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Piericidin A (Shaoguanmycin B, Antibiotic MT 1882-I, Antibiotic SN 198E, Antibiotic IT 143D, Piericidin A1)

Cat no: P4155

Piericidin A (Shaoguanmycin B, Antibiotic MT 1882-I, Antibiotic SN 198E, Antibiotic IT 143D, Piericidin A1)

Piericidin A is the major analogue of a family of pyridyl antibiotics isolated from selected Streptomyces sp. It is a specific, potent inhibitor of NADH-ubiquinone oxidoreductase (Complex I) that binds to ubiquinone binding site(s). Piericidin A inhibits both mitochondrial and bacterial NADH-ubiquinone oxidoreductases, binding close to NUOD-NUOB interface.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4155

Size

2mg

Form

Supplied as a pale yellow oil. Soluble in ethanol, methanol, DMF or DMSO.

Purity

~95% by HPLC

References

1. Evidence for a quinone binding site close to the interface between NUOD and NUOB subunits of \nComplex I. Prieur I. et al. Biochim. Biophys. Acta 2001, 1504, 173. \n2. -->H+/2e- stoichiometry in NADH-quinone reductase reactions catalyzed by bovine heart \nsubmitochondrial particles. Galkin A.S. et al. FEBS Lett. 1999, 451, 157. \n3. The 49-kDa subunit of NADH-ubiquinone oxidoreductase (Complex I) is involved in the binding of \npiericidin and rotenone, two quinone-related inhibitors. Darrouzet E. et al. FEBS Lett. 1998, 10, 34. \n4. Two binding sites of inhibitors in NADH: ubiquinone oxidoreductase (complex I). Relationship of one site \nwith the ubiquinone-binding site of bacterial glucose:ubiquinone oxidoreductase. Friedrich T. et al. Eur J \nBiochem. 1994, 219, 691.

Alternative Names

Shaoguanmycin B, Antibiotic MT 1882-I, Antibiotic SN 198E, Antibiotic IT 143D, Piericidin A1

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