PIK3R5 (Phosphoinositide 3-kinase Regulatory Subunit 5, PI3-kinase Regulatory Subunit 5, PI3-kinase p101 Subunit, Phosphatidylinositol-4,5-bisphosphate 3-kinase Regulatory Subunit, PtdIns-3-kinase Regulatory Subunit, Protein FOAP-2, PtdIns-3-kinase p101,

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110a, p110b, p110y, and p110d) have been isolated, and the regulatory subunits that associate with p110a, p110b, and p110d are p85a and p85b (3). In contrast, p110y associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110y is activated by BY subunits of heterotrimeric G proteins (4). The p101 regulatory subunit binds to Gby, released from heterotrimeric G proteins, and recruits bound p110y catalytic subunit to the plasma membrane, which is required for GPCR-induced PI3Ky activity (5).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4073-26P

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Cantley, L.C. (2002) Science 296, 1655-7. 2.Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. 3.Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. 4.Stoyanov, B. et al. (1995) Science 269, 690-3. 5.Voigt, P. et al. (2005) J Biol Chem 280, 5121-7.

Additional Info

Recognizes endogenous levels of total human PI3 Kinase p101. Species Crossreactivity: Mouse and rat.