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PIM1, Recombinant, Human, aa38-290, His-Tag (Non-specific Serine/threonine Protein Kinase, PIM)

Cat no: 137161

PIM1, Recombinant, Human, aa38-290, His-Tag (Non-specific Serine/threonine Protein Kinase, PIM)

PIM1 belongs to the Ser/Thr protein kinase family, and PIM subfamily. This protein is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons. \n\nSource: \nRecombinant protein corresponding to human Pim1, fused to His-tag at N-terminal expressed in E.coli.\n\nMolecular Weight: \n~31.4kD (274aa) (MALDI-TOF) \n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MYQVGPLLGS GGFGSVYSGI RVSDNLPVAI KHVEKDRISD WGELPNGTRV PMEVVLLKKV SSGFSGVIRL LDWFERPDSF VLILERPEPV QDLFDFITER GALQEELARS FFWQVLEAVR HCHNCGVLHR DIKDENILID LNRGELKLID FGSGALLKDT VYTDFDGTRV YSPPEWIRYH RYHGRSAAVW SLGILLYDMV CGDIPFEHDE EIIRGQVFFR QRVSSECQHL IRWCLALRPS DRPTFEEIQN HPWM\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137161

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 10% glycerol, 1mM DTT.

Purity

~90% (SDS-PAGE)

References

1. Koike N., et al. (2000) FEBS Lett. 467:17-21. 2. Ionov Y., et al. (2003) Anticancer Res. 23:167-178.

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