PKN (Serine/Threonine-protein Kinase N1, Protease-activated Kinase 1, PAK-1, Protein Kinase C-like 1, Protein Kinase C-like PKN, Protein Kinase PKN-alpha, Protein-kinase C-related Kinase 1, Serine-threonine Protein Kinase N, PKN1, PAK1, PRK1, PRKCL1)

Rho, the Ras-related small GTPase, is responsible for the regulation of actin based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins act as molecular switches, which are able to turn cytokinesis on and off. Although little is know about signaling downstream of Rho, several proteins have been implicated as Rho effectors. Protein kinase N (PKN) is a fatty acid-activated serine/threonine kinase whose catalytic domain exhibits homology with that of the PKC family. PKN associates with Rho via its amino terminus, is activated in a GTP-dependent manner and phosphorylates the head-rod domain of neurofilament protein. A second protein, rhophilin, exhibits 40% sequence identity with the amino terminal Rho binding domain. The enzymatic activity of rhophilin has not been demonstrated and it is possible that it acts through the recruitment of cytoskeletal components that initiate a kinase signaling cascade. Citron, a large protein with a predicted molecular weight of 183kD, interacts specifically with active Rho and Rac 1 but not Cdc42. Citron exhibits a distinctive protein organization and little homology with the Rho binding domains of PKN and rhophilin.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml for 2h at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat cells, pancreas\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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