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Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

Cat no: P4258-15

Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-BB is a 24.3kD B chain homodimer protein containing 109 amino acid residues.\n\nBiological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is 1-3ng/ml.\n\nResponding Cells (partial list): Fibroblasts, connective tissue\n\nConcentration Range for most in vitro applications: 0.2-10ng/ml\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, carrier protein. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

P4258-15

Size

2ug

Form

Supplied as a lyophilized powder from 10mM Acetic acid. No preservatives added. Reconstitute with ddH2Oand protein carrier to 0.1-1mg/ml. This solution can be diluted into other aqueous buffers.

Purity

(same/more than) 98% by SDS-PAGE and HPLC. Endotoxin: (same/less than) 0.1ng/ug.

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