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Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

Cat no: P4258-15C

Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

Recombinant protein expressed in yeast (S. cerevisiae) from the c-sis oncogene. \n\nSterility: Sterilized through a 0.2um ?lter and packaged aseptically.\n\nReconstitution: Dilution with 4mM HCl or 10mM acetic acid containing 1-2mg/ml BSA is recommended. \nNote: In dilute solution, PDGF adheres to surfaces, particularly glass. Therefore, use only polypropylene containers, pipettes and tips. Biological activity is destroyed upon reduction. \n\nBiological Activity: 5ng/ml of this lot of PDGF-BB supported a 1.9-fold increase in cell growth of NIH 3T3 ?broblasts over control cultures without PDGF-BB. The optimal PDGF concentration in cell culture can vary depending on cell type. \n\nPDGF Growth Assay:\n1. Prepare a cell suspension in 2% platelet poor serum to a ?nal concentration of 1x10e4 cells/ml. Aliquot 4ml/tube to seed quadruplicate wells (1ml/well) of test, positive and negative controls. \n2. Prepare PDGF-BB following instructions above for a concentration of approximately 1ug/ml and appropriate dilutions. \n3. Add PDGF-BB directly to the 4ml suspension, mix well and dispense 1ml/well (?nal concentration 0-10ng/ml). \n4. Refeed on day 3 with growth medium + PDGF-BB. \n5. Count and record cell numbers on day 5. Calculate the stimulation factor over negative control results.\n\nStorage and Stability: Lyophilized powder may be stored at -20 degrees C. May be stored at 4 degrees C for short-term only. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4258-15C

Size

10ug

Form

10ug PDGF-BB, lyophilized from 40ul of 0.1M acetic acid. Quantitated by amino acid analysis.

Purity

Puri?ed by HPLC. SDS-PAGE Analysis: Reduced PDGF-BB runs as one band at 16-17kDa. Non-reduced PDGFBB runs as one band at 30-32kDa. Because of anomalous behavior, PDGF-BB sometimes runs as a doublet at 14-18kDa.

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