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Platelet Derived Growth Factor Receptor beta (PDGFRb) phosphorylated (Tyr740)

Cat no: P4258-02F

Platelet Derived Growth Factor Receptor beta (PDGFRb) phosphorylated (Tyr740)

Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-alpha and PDGF receptor-beta. PDGF receptor-beta can only bind two specific PDGF isoforms, PDGF-beta and PDGF-delta (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Both Tyrosine 751 and 740 is required for PDGFR-beta mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (4).\n\nApplications: \nSuitable for use in Immunocytochemistry, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000 \nImmunocytochemistry: 1:100-1:250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

P4258-02F

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Deuel, T.F., et al. Biofactors 1: 213

Additional Info

Recognizes human PDGF-Receptor beta phosphorylated at Tyrosine 740. Species crossreactivity: mouse.

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