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Platelet Derived Growth Factor Receptor, Type B, CT (PDGFRb, CD140 antigen-like family member B, CD140b, CD140B, JTK12, PDGFR, PDGFR1, PDGF-R-beta)

Cat no: P4258-03C

Platelet Derived Growth Factor Receptor, Type B, CT (PDGFRb, CD140 antigen-like family member B, CD140b, CD140B, JTK12, PDGFR, PDGFR1, PDGF-R-beta)

Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-alpha and PDGF receptor-beta. PDGF receptor-beta can only bind two specific PDGF isoforms, PDGF-beta and PDGF-delta. Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase, Grb2, and Src, among others. \n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000\nImmunoprecipitation: 1:30\nImmunohistochemistry (Paraffin): 1:100\nImmunocytochemistry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nWestern Blot: NIH 3T3 cell lysate\nImmunohistochemistry: Prostate carcinoma, paraffin-embedded\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

P4258-03C

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Deuel, T.F., et al., Biofactors 1: 213

Additional Info

Recognizes human PDGF receptor beta, Mr 190kD. Does not crossreact with other CSF-1/PDGF receptor family members. Species Crossreactivity: Mouse.

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