Platelet Derived Growth Factor Receptor Type B (PDGFRb)

Two types of PDGF receptors have been identified: PDGF Receptor Type A (PDGFRa) and PDGF Receptor Type B (PDGFRb). The PDGF Receptor Type A, a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGF Receptor Type B, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity. Both receptors are transmembranous, ligand activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains. The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment.\n\nApplications:\nSuitable for use in Immunoblot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunoblot Analysis: 0.5-2ug/ml detected the PDGF Receptor Type B (~190kD) in RIPA lysates from mouse 3T3/A31 fibroblasts. The specificity of the immunoreaction was previously confirmed by using 1uM of the immunizing peptide to inhibit the antibody. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor Type B (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemilum-inescence detection system.\nImmunoprecipitation: 4ug immunoprecipitated the PDGF Type B Receptor from 500ug of 3T3/A31 RIPA lysate. \nImmunocytochemistry: 5-10ug/ml showed positive immunostaining for the PDGF Receptor Type B in 3T3/A31 cells fixed with 4% paraformaldehyde for 10 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Antigen Control: F4209\nAdd 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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