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Platelet Derived Growth Factor Receptor, Type B (PDGFRb) (PE)

Cat no: P4258-23B

Platelet Derived Growth Factor Receptor, Type B (PDGFRb) (PE)

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts, and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimer are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Two distinct PDGF receptors, the a-receptor and the b-receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the b-receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding. In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells, and neutrophils and has been reported to be important in the modification of cellular matrix constituents.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul labels 1x10e5 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

P4258-23B

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with R. Phycoerythrin (RPE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human Platelet Derived Growth Factor Receptor, Type B.

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