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PLCG1 (1-phosphatidylinositol-4,5-bisphosphate Phosphodiesterase gamma-1, PLC-148, Phosphoinositide Phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C-gamma-1, PLC-gamma-1, PLC1)

Cat no: P4074-43J

PLCG1 (1-phosphatidylinositol-4,5-bisphosphate Phosphodiesterase gamma-1, PLC-148, Phosphoinositide Phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C-gamma-1, PLC-gamma-1, PLC1)

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide-specific phospholipase C enzymes, C-beta is regulated by heterotrimeric G protein-coupled receptors, while the closely related C-gamma-1 (PLCG1) and C-gamma-2 enzymes are controlled by receptor tyrosine kinases. PLC gamma I and PLC gamma II are composed of phospholipase domains that flank regions of homology to noncatalytic domains of SRC, SH2 and SH3.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-10,000\nImmunohistochemistry (Paraffin): 1:100-250\nImmunocytochemistry: 1:100-250\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nWestern Blot: Ramos and Raji cell lysates\nImmunohistochemistry: Human kidney tissue \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

P4074-43J

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM tris-glycine, pH7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

References

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.

Additional Info

Recognizes human PLC gamma II at ~150kD.

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