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PLGF1, Recombinant, Human (Placental Growth Factor 1)

Cat no: P4273-10

PLGF1, Recombinant, Human (Placental Growth Factor 1)

Placenta growth factor (PlGF), a homodimeric glycoprotein that is homologous to vascular endothelial growth factor (VEGF), is mitogenic to endothelial cells and protects trophoblast from apoptosis. Alternative splicing of mature mRNA gives rise to three known isoforms of PlGF. PlGF is expressed by human trophoblast during normal pregnancy, however, it is not known which isoforms are produced.\n\nRecombinant Human Placenta Growth Factor-1(PIGF/PGF) produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of 29.7kD. Recombinant Human PLGF-1 is purified by proprietary chromatographic techniques\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ala-Val.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nRecombinant Human PLGF-1 is fully biologically active when compared to standard. The ED50 as determined by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant human PlGF-1 can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5

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SPECIFICATIONS

Catalog Number

P4273-10

Size

2ug

Form

Supplied as a lyophilized powder containing BSA.

Purity

(same/more than) 90% as determined by: \n(a) Analysis by RP-HPLC. \n(b) Anion-exchange FPLC. \n(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

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