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PMM1, Recombinant, Human (PMM 1, PMMH-22, Phosphomannomutase 1, PMMH22)

Cat no: P4296-10

PMM1, Recombinant, Human (PMM 1, PMMH-22, Phosphomannomutase 1, PMMH22)

PMM1 (Phosphomannomutase 1) is an enzyme that involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. This enzyme catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. In addition, it may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.\n\nSource:\nRecombinant corresponding to aa1-262 of human PMM1 protein, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI \nEKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE \nKIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC \nREIFFPETAH EA\n\nMolecular Weight: 31.9kD (282aa) confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4296-10

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol, 2mM DTT, 100mM sodium chloride, 0.1mM PMSF.

Purity

~90% (SDS-PAGE).

References

1. Veiga-da-Cunha M., et al. (2008) J Biol Chem. 283(49):33988-93. 2. Silvaggi N.R., et al. (2006) J. Biol. Chem. 281:14918-14926

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