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Pneumolysin

Cat no: P4332-10

Pneumolysin

Pneumolysin (pneumococcal hemolysin) is an intracellular protein produced by all strains of S. pneumoniae (1) and is a cytoplasmic cholesterol-dependent cytolysin forming large pores in cholesterol-containing membranes and are therefore cytotoxic to mammalian cells (2). The molecular weight of Pneumolysin is approximately 53kD.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4332-10

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a liquid in 0.01M phosphate buffer, pH 7.4, 0.5M NaCl and 15mM sodium azide.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.\n

Isotype

IgG1,k

References

1. Kalin M, Kanclerski K, Granstrom M, Mollby R (1987) Diagnosis of Pneumococcal Pneumonia by Enzyme-Linked Immunosorbent Assay of Antibodies to Pneumococcal Hemolysin (Pneumolysin). J Clin Microbiol 25:226-229. 2. Gilbert RJC (2002) Pore-forming toxins. Cell Mol Life Sci 59:832-844.

Additional Info

Recognizes Pneumolysin.

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