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PNMT (Phenylethanolamine N Methyltransferase)

Cat no: P4350-06

PNMT (Phenylethanolamine N Methyltransferase)

Phenylethanolamine-N-methyltransferase (PNMT) is an enzyme converting noradrenaline to adrenaline. The enzyme is present in adrenomedullary cells and in the brain neurons. \n\nApplications:\nSuitable for use in Immunohistochemistry (frozen and paraffin sections). Other applications not tested.\n\nRecommended Dilution: \nImmunohistochemistry: 1:400-1:800 (PAP) with overnight incubation at 4 degrees C.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nDEPC-fixed paraffin sections of rat adrenal gland.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, dH2O. Reconstituted product is stable for 12 months at -20 degrees C Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4350-06

Size

50ul

Applications

IHC

Reactivities

Rat, Bov

Form

Supplied as a lyophilized powder in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Reconstitute in 100ul sterile 40-50% glycerol, dH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

References

Bjartell A et al. Helodermin-like peptides in noradrenaline cells of adrenal medulla Reg Pept 26:27-34 (1989).

Additional Info

Recognizes bovine PNMT. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining. Species Crossreactivity: rat.

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