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PNP, aa1-289, Recombinant, Human (Purine Nucleoside Phosphorylase, Inosine Phosphorylase, NP)

Cat no: P4350-10A

PNP, aa1-289, Recombinant, Human (Purine Nucleoside Phosphorylase, Inosine Phosphorylase, NP)

Purine Nucleoside Phosphorylase (PNP) catalyzes the phophorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. Physiological substrates of PNP include inosine, guanosine, and 2'-deoxyguanosine, but not adenosine (1). PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T-lymphocyte function and neurologic dysfunction (2, 3). PNP is used in assays for the measurement of inorganic phosphate (4).\n\nSource: \nRecombinant corresponding to aa1-289 from human PNP, fused with 6-His tag at C-terminal, expressed in E. coli.\n\nMolecular Weight: \n~33kD\n\nEndotoxin Level:\n(same/less than)1EU/1ug (LAL)\n\nBiological Activity:\nMeasured by the phosphorolysis of 7-methyl-6-thioguanosine. The specific activity is >35,000 pmoles/min/ug, as measured under the described conditions.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4350-10A

Size

10ug

Form

Supplied as a liquid in Tris, sodium chloride.

Purity

~95% (SDS-PAGE)

References

1. Schramm, V.L. (1998) Annu. Rev. Biochem. 67:693. 2. Stoop, W. et al. (1977) N. Eng. J. Med. 296:651. 3. Markert, M.L. (1991) Immunodefic. Rev. 3:45. 4. Webb, M.R. (1992) Proc. Natl. Acad. Sci. USA. 89:4884.

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