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POFUT1, Recombinant, Human, aa27-388, His-Tag (GDP-fucose Protein O-fucosyltransferase 1, FUT12, O-Fuc-T, O-FucT-1, O-FUT)

Cat no: 138713

POFUT1, Recombinant, Human, aa27-388, His-Tag (GDP-fucose Protein O-fucosyltransferase 1, FUT12, O-Fuc-T, O-FucT-1, O-FUT)

GDP-fucose protein O-fucosyltransferase 1, also known as POUFUT1, is a member of the glycosyltransferase O-Fuc family. Highly expressed in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle, POFUT1 uses manganese to catalyze the attachment of fucose to a conserved serine or threonine residue on a protein accept. POFUT1 plays an important role in Notch signaling, as Notch ligands can serve as POFUT1 substrates. \n\nSource:\nRecombinant protein corresponding to aa27-388 from human POFUT1 at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~43.7kD (385aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSGSWDPAG YLLYCPCMGR FGNQADHFLG SLAFAKLLNR TLAVPPWIEY QHHKPPFTNL HVSYQKYFKL EPLQAYHRVI SLEDFMEKLA PTHWPPEKRV AYCFEVAAQR SPDKKTCPMK EGNPFGPFWD QFHVSFNKSE LFTGISFSAS YREQWSQRFS PKEHPVLALP GAPAQFPVLE EHRPLQKYMV WSDEMVKTGE AQIHAHLVRP YVGIHLRIGS DWKNACAMLK DGTAGSHFMA SPQCVGYSRS TAAPLTMTMC LPDLKEIQRA VKLWVRSLDA QSVYVATDSE SYVPELQQLF KGKVKVVSLK PEVAQVDLYI LGQADHFIGN CVSSFTAFVK RERDLQGRPS SFFGMDRPPK LRDEF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138713

Size

100ug

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 2M urea, 10% glycerol.

Purity

~85% (SDS-PAGE)

References

1. Wang Y., et al. (2001) J Biol Chem. 276:40338-40345. 2. Luo Y., et al. (2005) J Biol Chem. 280:11289-11294.

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