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Products PPAR(gamma) Transcription Factor Assay Kit
| Catalogue number: | 10006855 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|Nuclear Receptors|PPAR|Intracellular Signaling |
| Shipping temp: | -80 |
| Storage temp: | -80 |
| Additional info: | Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPAR.alpha., .beta./.delta., and .gamma., which are expressed at variable levels in different tissues. The Cayman Chemical PPAR.gamma. Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPAR.gamma. binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPAR.gamma. is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. |
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