PPP6C, NT (Serine/Threonine Protein Phosphatase 6 Catalytic Subunit, PP6)

PPP6C belongs to the PPP phosphatase family, PP-V subfamily. Reversible phosphorylation of proteins on serine and threonine residues is an important biochemical event that regulates a broad variety of intracellular processes. The phosphorylation state is determined by the well-controlled balance of activities of serine/threonine-specific protein kinases and protein phosphatases, including PPP6C. Expression levels are highest in testis, heart, and skeletal muscle and lowest in placenta, lung, and kidney. PPP6C can complement mutations in the S. cerevisiae Sit4 and S. pombe ppe1 genes, indicating that PPP6C is the functional homolog of yeast Sit4p and ppe1. Since Sit4p is required for the G1 to S transition of the cell cycle and ppe1 is involved in cell shape control and mitotic division, it has been suggested that PPP6C functions in cell cycle regulation.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9107-56N

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Yang, J., et al., EMBO J. 24(1):1-10 (2005). 2.Zhou, G., et al., J. Biol. Chem. 279(45):46595-46605 (2004). 3.Huang, S., et al., J. Biol. Chem. 279(35):36490-36496 (2004). 4.Swingle, M.R., et al., J. Biol. Chem. 279(32):33992-33999 (2004). 5.Wechsler, T., et al., Proc. Natl. Acad. Sci. USA 101(5):1247-1252 (2004).

Additional Info

Recognizes human PPP6C.