PRDX1 (Natural killer cell-enhancing factor A)

PRDX1(Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical \nroles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and \nact as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of \nantioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The PRDX1 gene is \nmapped on 1p34.1. Prdx1 was expressed in differentiating motor neuron cells in developing embryonic \nchicken and mouse spinal cords. mmunoprecipitation analysis showed that GDE2 interacted directly \nwith PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells. This protein \nmay have a proliferative effect and play a role in cancer development or progression. In differentiating \nspinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between \nthe Gde2 N- and C-terminal domains. An intramolecular disulfide bond between the GDE2 N- and \nC-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 \nfor the induction of motor neuron differentiation.\n\nUniProt Number:\nQ06830\n\nGene ID:\nPRDX1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145081

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Neumann, C. A., Krause, D. S., Carman, C. V., Das, S., Dubey, D. P., Abraham, J. L., Bronson, R. T., \nFujiwara, Y., Orkin, S. H., Van Etten, R. A.Essential role for the peroxiredoxin Prdx1 in erythrocyte \nantioxidant defence and tumour suppression. Nature 424: 561-565, 2003.\n2. Prosperi, M.-T., Apiou, F., Dutrillaux, B., Goubin, G. Organization and chromosomal assignment of \ntwo human PAG gene loci: PAGA encoding a functional gene and PAGB a processed \npseudogene. Genomics 19: 236-241, 1994.\n3. Yan, Y., Sabharwal, P., Rao, M., Sockanathan, S. The antioxidant enzyme Prdx1 controls neuronal \ndifferentiation by thiol-redox-dependent activation of GDE2. Cell 138: 1209-1221, 2009.

Additional Info

Recognizes human, mouse and rat PRDX1. No crossreactivity with other proteins.