PRMT5 (Protein arginine N Methyltransferase 5, Shk1 Kinase-binding Protein 1 Homolog, Jak-binding Protein 1, JBP1)

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of omega-N(G)-monomethylarginine and symmetric omega-N(G),N(G')-dimethylarginine in a variety of proteins. Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein. PRMT5 appears to have lower specific enzyme activity than PRMT1.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1,000\nHeLa cell lysate can be used as positive control.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9004-28

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 200ul of PBS containing 0.02% sodium azide and 0.1 mg/ml BSA.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Chen D, Ma H, Hong H, Koh SS, Huang SM, Schurter BT, Aswad DW, and Stallcup MR. Science 284, 2174-2177 (1999). 2. Rho J, Choi S, Seong YR, Cho WK, Kim SH, Im DS. J Biol Chem 276(14):11393-11401 (2001). 3. Branscombe TL, Frankel A, Lee JH, Cook JR, Yang Z, Pestka S, Clarke S. J Biol Chem 276(35):32971-32976 (2001). 4. Fabbrizio E, El Messaoudi S, Polanowska J, Paul C, Cook JR, Lee JH, Negre V, Rousset M, Pestka S, Le Cam A, Sardet C. EMBO Rep 3(7):641-645 (2002).

Additional Info

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of omega-N(G)-monomethylarginine and symmetric omega-N(G),N(G')-dimethylarginine in a variety of proteins. Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein. PRMT5 appears to have lower specific enzyme activity than PRMT1.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1,000\nHeLa cell lysate can be used as positive control.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C or colder. Aliquots are stable for at least 24 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.