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PRMT6 (Protein Arginine N-methyltransferase 6, Histone-arginine N-methyltransferase PRMT6, Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-like Protein 6, HRMT1L6, PRMT-6)

Cat no: P9004-30G

PRMT6 (Protein Arginine N-methyltransferase 6, Histone-arginine N-methyltransferase PRMT6, Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-like Protein 6, HRMT1L6, PRMT-6)

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:100\nImmunocytochemistry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9004-30G

Size

50ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide, 0.1mg/ml BSA.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human PRMT6. Species sequence homology: Mouse.

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