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Prostaglandin D2-MOX Express EIA Kit

Cat no: 500151

Prostaglandin D2-MOX Express EIA Kit

Cayman's PGD2-MOX Express EIA is a competitive assay that permits the rapid measurement of PGD2 from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in the PGD2-MOX EIA (Item No. 512011). The EIA offers the convenience of a fast assay while still achieving an IC50 value of 75 pg/ml and a detection limit (80% B/B0) value of approximately 16 pg/ml.

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SPECIFICATIONS

Catalog Number

500151

Form

96 Well

P Type

Assay Kits|Prostaglandins|Eicosanoids

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Prostaglandin D2 (PGD2) is biosynthesized in the brain by a lipocalin-type PGD2 synthase where it acts in the CNS to promote sleep induction and lowering of body temperature. PGD2 is also the major eicosanoid product of mast cells and is synthesized in large quantities by a hematopoetic-type PGD synthase during allergic and asthmatic anaphylaxis. Measurement of the parent eicosanoid PGD2 is appropriate in the supernatants of cell cultures, where PGD2 levels may reach several ng/ml, and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman Chemical's PGD2-MOX Express EIA is a competitive assay that permits the rapid measurement of PGD2 from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in Cayman's widely utilized PGD2-MOX EIA (Catalog No. 512011). While Catalog No. 512011 offers superior sensitivity (IC50 ~20 pg/ml), the new PGD2-MOX Express EIA offers the convenience of a fast assay while still achieving an IC50 of 75 pg/ml and a detection limit (80% B/B0) of approximately 16 pg/ml.

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