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Proteasome 26S, P52 Subunit

Cat no: P9078-65

Proteasome 26S, P52 Subunit

The p52 subunit (Mr 52,000) is the major antigen of the 19S cap complex, which is a subcomplex of the 26S proteasome, implicated in ATP- and ubiquitin-dependent protein degradation.\n\nApplications:\nSuitable for use in Western Blot. Not recommended for Immunofluorescence and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:5-1:10 when using the ECL method. Dilution buffer: 0.15M PBS with 0.1% BSA and 0.1% sodium azide. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9078-65

Size

5ml

Applications

WB

Hosts

Mouse

Reactivities

Hum, Rat, Xen/Amph

Form

Supplied as a liquid containing 0.09% sodium azide

P Type

Mab

Purity

Hybridoma culture supernatant

Isotype

IgG1

References

Peters JM, Franke WW, and JA Kleinschmidt: distinct 19S and 20S subcomplexes of the 26S proteasome and their distribution in the nucleus and the cytoplasm. J Biol Chem 269:7709-7718 (1994)\nJM Peters: Proteasomes: protein degradation machines of the cell. TIBS 19, September 1994

Additional Info

Recognizes Proteasome 26S, P52 Subunit. Species Crossreactivity: Xenopus laevis; XLKE-A6 (X. laevis). Cultured Cell Lines: PLC, A-431, MCF-7, CaCo (human); RVF (rat); Ptk2 (kangaroo rat). Does not react with 3T3 (mouse).

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