Protein Gene Product 9.5 (PGP9.5, UchL1, Ubiquitin C-terminal Hydrolase, PARK5)

PGP9.5 (protein gene product 9.5), also known as UchL1 (ubiquitin C-terminal hydrolase) and PARK5, is a neuronal biomarker and ubquitin system protein (reviewed in Day and Thompson, 2010). PGP9.5 is a highly conserved protein and has shown that it is expressed in neurons and neuroendocrine cells in vertebrates where it comprises about 5-10% of soluble cytoplasmic proteins. A minor proportion of PGP9.5 in brain is tightly bound to the membrane. PGP9.5 is also expressed in human oocytes and spermatogonia. It is important to note that the use of an antibody as a biomarker in this case does not imply that an antibody defines absolute tissue specificity. Rather, it means that PGP9.5 is expressed in neurons and neuroendrocine cells at significantly higher levels than in other cell types. A mutation in the UchL1 gene has been found to cause a form of Parkinson's disease, and this discovery has spurred considerable research interest in UchL1 and to its alternative name of PARK5. Antibody to UchL1 is widely used as an immunohistochemical marker of nerves and neuroendocrine cells.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: pairs with 138591\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138590

Size

20ug

Applications

ELISA

Hosts

Mouse

Reactivities

Mouse, Rat, Bov, Can, GP, Prc, Rabbit, Sheep, ZF/Fish

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

1. Day INM and RJ Thompson. Prog Neurobiol 90:327-352 (2010). The distribution of PGP9.5 is extensively described in this review article, researchers are encouraged to consult this outstanding article for additional information. 2. Gaunitz C et al. J Food Protection 72:1070-1077 (2009)). Sandwich ELISA (cow and pig meat). PGP9.5 antibodies from clones 13C4 and 31A3 were used to detect neuronal contaminant components in meat. Refer to the publication for additional details.

Additional Info

Recognizes human UchL1. Species Crossreactivity: bovine, canine, guinea pig, mouse, porcine, rabbit, rat, sheep, zebrafish