Protein Kinase G1 beta (PKG1 beta, Cyclic GMP-dependent Protein Kinase)

Cyclic GMP-dependent protein kinases (PKGs or cGKs) are classified into two types, PKGI and PKGII based on their historical order of characterization. There are two PKGI isoforms, PKGIalpha (76kD) and PKGIbeta (78kD) which arise from the alternative splicing of a single gene. These two isoforms differ in their amino-terminal autoinhibitory domains but are similar in their cGMP-binding sites and catalytic domains. PKGIalpha is highly expressed in the Purkinje cell of the cerebellum, in platelets, lung and in smooth muscle cells whereas the PKGIbeta isoform is primarily expressed in smooth muscle cells (1,2). Studies have shown that cGKs and cyclic AMP-dependent kinases (cAKs) are highly homologous protein kinase families with similar substrate specificities and phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility and platelet aggregation (3,4,5,6). However, in most of these studies a clear dissection between the cGMP kinase pathway and other pathways such as the cAMP kinase pathway have been difficult to achieve. Recent studies using mice deficient in PKGs have shown that cGMP kinases regulate very specifically distinct pathways which are separate from those used by cyclic AMP kinases (7). \n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot (ECL): 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nHeLa Cell Lysate and Rat Brain Tissue Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9102-95

Size

50ug

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Collins, S.P. and Uhler, M.D. (1999) J. Biol. Chem 274: 8391-8404. 2. Keilbach, A. et al. (1992) Eur. J. Biochem. 208: 467-473. 3. Francis, SH. and Corbin, J.D. (1994) Adv. Pharmacol. 26: 115-170. 4. Mcknight, G.S. (1991) Curr. Opin. Cell. Biol. 3: 213-217. 5. Walsh, DA. and Van Patten, SM. (1994) FASEB J 8: 1227-1236. 6. Kuo, JF. (1975) Proc. Natl. Acad. Sci. USA. 72: 2256-2259. 7. Ruth, P. (1999) Pharmacol. Ther. 82: 355-372. 8. Thompson, W.J., Piazza, G.A., Li, H., Liu, L., Fetter, J., Zhu, B., Sperl, G., Ahnen, D., Pamukcu, R. (2000) Cancer Res 60(13): 3338-3342. 9. Tao, Y.X., Hassan, A., Haddad, E., Johns, R.A. (2000) Neuroscience 95(2): 525-533. 10. Cook, A.L.M. Frydenberg, M., Haynes, J.M. (2002) Cell Signal 14(12): 1023-1029. 11. Gerzanich, V., Ivanov, A., Ivanova, S., Yang, J.B., Zhous, H., Dong, Y., Simard, J.M. (2003) Circ Research 93(9): 805-812.

Additional Info

Recognizes human PKG1beta at 80kD. Species Crossreactivity: mouse, rat, bovine.