Protein MW Marker, Ladder, Prestained, 10-170kD

Prestained Protein Ladder is a 3-color protein molecular weight standard containing 10 prestained recombinant proteins covering a wide range molecular weights from 10-170kD. The ladder contains one orange reference band of ~70kD and one green reference band of 10kD.\nLot-to-lot variation of the apparent molecular weight of prestained proteins is ~5%. The ladder is supplied in gel loading buffer and is ready-to-use: no heating, further dilution or addition of a reducing agent is required. Prestained Protein Ladder for 100 mini gel applications 5ul per well or 50 large gel applications 10ul per well.\n\n5ul of Prestained Protein Ladder provide 10 bands of equal intensities in 4-20% SDS-PAGE (Tris-glycine buffer) and after electrotransfer onto PVDF membrane.\n\nApplications:\n

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SPECIFICATIONS

Catalog Number

P9106-51

Size

250ul

Applications

WB

References

1. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685, 1970.\n2. Burnette, W.N., \"Western blotting\": electrophoretic transfer of proteins from sodium dodecyl sulfate - polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal Biochem., 112 (2), 195-203, 1981.\n3. Towbin, H., et al., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350-4354, 1979.