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PSMA2 (Proteasome Subunit alpha Type 2, Proteasome Component C3 and Macropain Subunit C3, HC3, MU, PMSA2, PSC3)

Cat no: 145586

PSMA2 (Proteasome Subunit alpha Type 2, Proteasome Component C3 and Macropain Subunit C3, HC3, MU, PMSA2, PSC3)

PSMA2 (Proteasome subunit alpha type 2; also proteasome component C3 and Macropain subunit C3) is an intracellular 25-27kD member of the peptidase T1A family of molecules. It is ubiquitously expressed, and participates in the formation of the 700kD 20S proteasomal complex. This complex contains four stacked rings, forming a barrel. The top and bottom rings are made of seven alpha-subunits, while the two central rings are composed of seven catalytic beta-subunits. The complex degrades 80-90% of all intracellular proteins through both a ubiquitin-dependent and -independent process. PSMA2 may modulate the activity of select beta-subunits. Human PSMA2 is 234 amino acids (aa) in length. It contains at least five utilized phosphorylation sites plus one nitrated tyrosine. Full-length human PSMA2 shares 99% aa sequence identity with mouse PSMA2.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145586

Size

100ug

Applications

WB

Hosts

Sheep

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.

P Type

Pab

Purity

Purified by Protein A or G affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human, mouse and rat PSMA2 in Western blots.

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