Purified anti-Sp3

The sequence-specific transcription factor Sp3 binds GC and GT box sequences in the promoters of a variety of genes. The Sp3 transcription factor contains three zinc finger domains that determine sequence specificity of DNA binding. This transcription factor is thought to regulate nucleoside, nucleotide and nucleic acid metabolism and many other "housekeeping" genes. Sp3 can be modified by sumoylation by Ubc9 and has been shown to interact with other transcription factors and histonbe deacetylases. Sp3 has been reported to act as a transcriptional enhancer and repressor of Sp1 activity in a cell- and context-dependent manner. The Poly6248 antibody recognizes the C-terminal region of human, mouse, and rat Sp3 and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

624802

Size

200 microl

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Clone

Poly6248

Antigen

Peptide mapping to the carboxy terminus of human Sp3

Gene Id

6670

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at -20C.

Alternative Names

Transcription factor Sp3, SP3

Regulatory Status

RUO