Pyruvate dehydrogenase (PDHX, E3BP, OPDX, PDX1, proX, DLDBP, pyruvate dehydrogenase complex, component X, E3-binding protein, pyruvate dehydrogenase complex, lipoyl-containing component X) (Control Peptide)

C-KSFKANLENPIRLA, C Terminus\nControl peptide corresponding to polyclonal antibody, Catalog#P9544-01, goat x human.\n\nApplication(s):\nSuitable for use in Peptide ELISA. Other applications have not been tested. \n\nRecommedned Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking (see corresponding antibody P9544-01):\nAntibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

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SPECIFICATIONS

Catalog Number

P9544-01-P

Size

100ug

Applications

ELISA

Form

Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.

Purity

Highly Purified

References

Li Y, Cao X, Li LX, Brubaker PL, Edlund H, Drucker DJ. , {beta}-Cell Pdx1 Expression Is Essential for the Glucoregulatory, Proliferative, and Cytoprotective Actions of Glucagon-Like Peptide-1., Diabetes. 2005 Feb;54(2):482-91.