RAD18 (hRad18)

Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (1, 2). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (3). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (1, 2). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (4). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56kD. The human Rad18 is ubiquitously expressed in various human tissues. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nHL60 cells can be used as positive control and a band of approx. size of 60 kD should be detected. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R0445-25

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Tateishi S, et al., Proc. Natl. Acad. Sci. USA 97: 7927-7932 (2000). 2. Xin H, et al., Nucleic Acids Res 28: 2847-2854 (2000). 3. Haynes, RH et al., (Cold Spring Harbor Lab. Press, Plainview, NY). Vol. 1, pp. 371-414 (1981). 4. Bailley V, et al., Genes Dev. 8: 811-820 (1994). 5. Lyakhovich, A., et al., Cell Biol. 23 2463-2475 (2003).

Additional Info

Recognizes human RAD18 (hRad18)