Rad21 (Double Strand Break Repair Protein Rad21 Homolog, FLJ25655, FLJ40596, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear Matrix Protein 1, NXP-1, NXP1, RAD21 Homolog, SCC1, SCC1 Homolog)

The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2 or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). \n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% \nTween-20 at 4 degrees C with gentle shaking, overnight. \nImmunoprecipitation: 1:25.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

R0447-02B

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied in 10 mM sodium HEPES pH 7.5, 150 mM NaCl, 100 microg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114, 2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35, 3. Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67, 4. Hauf, S. et al. (2005) PLoS Biol 3, e69, 5. Hauf, S. et al. (2001) Science 293, 1320-3, 6. Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77, 7. Chen, F. et al. (2002) J Biol Chem 277, 16775-81.

Additional Info

Recognizes Human RAD21. Species crossreactivity: Mouse, Rat Monkey. Sequence Homology: chicken, bovine, xenopus, horse.