RANTES, Recombinant, Mouse (Regulated on Activation, Normal T-cell Expressed and Secreted, CCL5)

Source: \nRecombinant corresponding to mouse RANTES expressed in E. coli.\n\nMolecular Weight: \n~8kD\n\nEndotoxin: ~0.1ng/ug\n\nBiological Activity: \nED50 range=5.0-20ng/ml, determined by the dose dependent chemotaxis of THP-1 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1100-07A

Size

10ug

Form

Supplied as a lyophilized powder. BSA free. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Purity

~95% (SDS-PAGE)

References

1. Schall, T.J., N.J. Simpson, and J.Y. Mak (1992) Molecular cloning and expression of the murine RANTES cytokine: Structural and functional conservation between mouse and man. Eur. J. Immunol. 22:1477-1481. 2. Haelens, A., A. Wuyts, P. Proost, S. Struyf, G. Opdenakker, and J. van Damme (1996) Leukocyte migration and activation by murine chemokines. Immunobiology 195:499-521. 3. Fischer, F.R., Y. Luo, M. Luo, L. Santambrogio, and M. Dorf (2001) RANTES-induced chemokine cascade in dendritic cells. J. Immunol. 167:1637-1643. 4. Zhang, Y., Q. Zhai, Y. Luo, and M. Dorf (2002) RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK). J. Biol. Chem. 277:19042-19048. 5. Luo, Y., M. Berman, Q. Zhai, F. Fisher, S.R. Abromson-Leeman, Y. Zhang, W.A. Kuziel, C. Gerard, and M.E. Dorf (2002) RANTES stimulates inflammatory cascades and receptor modulation in murine astrocytes. Glial 39:19-30.