This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, alpha (NR1B1), a ligand-dependent transcription factor commonly referred to as RARA or RAR(alpha). INDIGO's reporter cells include the luciferase reporter gene functionally linked to a RAR(alpha)-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in RAR(alpha) activity. The principal application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human RAR(alpha). RAR(alpha) reporter cells are prepared using INDIGO
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