The Rat Fibronectin ELISA kit is designed for detection of rat fibronectin in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures fibronectin in less than 4 hours. A polyclonal antibody specific for fibronectin has been pre-coated onto a microplate. Fibronectin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for fibronectin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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