RbAp48 (Retinoblastoma Binding Protein p48, CAF1 p48, Chromatin Assembly Factor 1 Subunit C, Histone Binding Protein RbBP4, Human Chromatin Assembly Factor-I p48 Subunit, MSI1 Protein Homolog, Nucleosome Remodeling Factor Subunit RBAP48, NURF-48, NURF55,

RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin \nremodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. RBBP4 is a component of several complexes which regulate chromatin metabolism. These complexes include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair as well as the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression (1) The growth suppression function of the retinoblastoma protein (Rb) is thought to be mediated by Rb binding to cellular proteins. Rb \ninteracts with multiple cellular proteins that mediate its cellular function. p48 is one of the major proteins that binds to a putative functional domain at the carboxy terminus of the Rb protein (2). Interaction with p48 may be one of the mechanisms for suppression of growth mediated by Rb. \nRbAp48 (RBBP4) and RbAp46 are quantitatively the major Rb-associated proteins. Results suggest that RBBP4 and RbAp46 may have shared as well as unique functions in the \nregulation of cell proliferation and differentiation (3).\n \nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50 \nWestern Blot: 1:10,000-1:50,000 \nImmunoprecipitation: 1:50 \nImmunohistochemistry: 1:100-1:250 \nImmunocytochemistry: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

R1200-62F

Size

100ul

Applications

FC, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008) 2. Qian YW et al. J Biol Chem 270(43):25507-13, 1995 3. Qian YW et al. Nature 364(6438):648-52, 1993 \n

Additional Info

Recognizes human RBBP4. Species Crossreactivity: Mouse and rat.