RBC Lysis/Fixation Solution (10X)

BioLegend RBC Lysis/Fixation Solution has been designed and optimized for one-step lysing of red blood cells (RBCs) and fixation of the remaining blood elements (such as leukocytes) following immunofluorescent staining with fluorochrome-conjugated antibodies. It can be used for either lyse/wash or lyse/no wash immunofluorescent staining procedures. This solution is supplied as a 10X concentrate. Dilute to 1X working solution with room temperature dI H20prior to use.

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SPECIFICATIONS

Catalog Number

422401

Size

50 ml

Applications

FC

Dilutions

Whole Blood Lyse/Wash Procedure:1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts of room temperature dI H20. 2. Mix 100 microl of whole blood with fluorochrome-conjugated antibody. 3. Incubate at room temperature, in the dark, for 20 minutes. 4. Add 2.0 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately. 5. Incubate at room temperature, in the dark, for 15 minutes. 6. Centrifuge at 350Xg for five minutes. Discard supernatant. 7. Add 2 mL BioLegend Cell Staining Buffer (Cat No 420201). 8. Centrifuge at 350Xg for five minutes. Discard supernatant. 9. Resuspend cell pellet in 0.3-0.5 mL Cell Staining Buffer. 10. Analyze cells immediately by flow cytometry or store at 4C, in the dark, for no longer than 12 hours. Whole Blood Lyse/No Wash Procedure:1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts room temperature dI H20. 2. Mix 50 microl of whole blood with fluorochrome-conjugated antibody. 3. Incubate at room temperature, in the dark, for 20 minutes. 4. Add 0.45 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately. 5. Incubate at room temperature, in the dark, for 15 minutes. 6. Analyze samples by flow cytometry.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Store RBC lysis buffer at 4C.

Alternative Names

Fix/Lyse, FACS Lysing Solution

Regulatory Status

RUO